Morphological lipofuscin was quantified in laboratory cultured Homarus gammarus to determine if the amount of pigment was correlated with age and culture temperature in this species. Two cohorts were monitored for 34 and 18 months, respectively. An age dependent accumulation of lipofuscin concentration (expressed as % area fraction) occurred (Y = -2.22 + 0.084X, R 2 = 46.8%, p ≤ 0.0001 and incorporating carapace length as a covariate). Carapace length accounted for 3.3% of the variation in lipofuscin. The effect of temperature on lipofuscin accumulation was assessed using three regimes: 8, 11 and 13.5±5°C. Temperature had a significant effect on lipofuscin accumulation (F = 15.9, p ≤ 0.0009). Carapace length also responded significantly to temperature although the trend was not linear (F = 68.1, p < 0.0001). There was no significant correlation between lipofuscin concentration and body size in lobsters of the same age (r = 0.067, -0.2, 0.154 and 0.5 for each of four groups). The age dependent increase in morphological lipofuscin and the lack of correlation between body size and lipofuscin in cultured H. gammarus indicates significant potential for this technique as a method of ageing lobsters. As lipofuscin accumulation is a function of metabolic age, factors other than temperature, such as population density, which affects growth rate in the wild, could potentially affect metabolic rate and therefore lipofuscin accumulation. However the independence between growth and lipofuscin in laboratory animals indicates that this may not be a problem.