The chromatographic behaviour of Hg(II), methylmercury, phenylmercury and Cu(II) on three different sorbents, strong acidic sulfobutyl cation-exchanger, weak basic aminopropyl anion-exchanger and silicagel has been studied. A dithizone solution in cetyltrimethylammonium hydrogensulfate water micellar medium was found as a useful post-column derivatization reagent for UV-VIS detection. The detection limits 1.1 ng, 2.2 ng, 6.2 ng and 1.6 ng were found in silicagel chromatographic system and dithizone detection system for Hg(II), methylmercury, phenylmercury and respectively for Cu(II).