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Nitric oxide production, l-arginine transport and intracellular [Ca 2+ ] changes in human platelets stimulated without stirring by low doses of collagen have been evaluated. Collagen decreased in a dose-dependent manner the nitric oxide formation. A reduction of about 30% of the basal level was produced by 5μg/mL. Aspirin did not change the collagen effect. The inhibition was reversed by EGTA...
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