Polar mesospheric clouds form in the summer high latitude mesopause region and are primarily comprised of H2O ice, forming at temperatures below 150K. Average summertime temperatures in the polar mesosphere (78°N) are approximately 125K and can be driven lower than 100K by gravity waves. Under these extreme temperature conditions and given the relative mesospheric concentrations of CO2 and H2O (~360ppmv and ~10ppmv, respectively) it has been hypothesised that CO2 molecules could become trapped within amorphous mesospheric ice particles, possibly making a significant contribution to the total condensed volume. Studies of CO2 trapping in co-deposited gas mixtures of increasing CO2:H2O ratio (deposited at 98K) were analysed via temperature programmed desorption. CO2 trapping was found to be negligible when the H2O flux to the surface was reduced to 4.8×1013 molecules cm−2s−1. This corresponds to an average of 0.4 H2O molecules depositing on an adsorbed CO2 molecule and thereby trapping it in amorphous ice. Extrapolating the experimental data to mesospheric conditions shows that a mesospheric temperature of 100K would be required (at a maximum mesospheric H2O concentration of 10ppmv) in order to trap CO2 in the ice particles. Given the rarity of this temperature being reached in the mesosphere, this process would be an unlikely occurrence.