Efficient tools for consistent species identification are essential to provide information about the implication of wildlife in forensic-related events. The objective of this study was to determine potential markers on the mitochondrial cytochrome b (CYTB), cytochrome c oxidase subunit I (COX1) and 16S rRNA genes for discriminating between three important cynegetic species. Samples included deer (Cervus elaphus), roe bucks (Capreolus capreolus) and wild boars (Sus scrofa). PCR amplification was performed with in-house designed primers followed by separation via capillary electrophoresis. The sequences were edited with Chromas Pro 1.34 and aligned with Clustal X v2.0. Consensus sequences of CYTB, COXI and 16S rRNA genes revealed interspecific mtDNA (p<0.05) to discriminate each of the three game species.