A new microfossil based biostratigraphy of the Paleocene and Lower Eocene sediments of the Hellefisk-1, Ikermiut-1, Kangamiut-1, Nukik-1, and Nukik-2 boreholes offshore West Greenland has been established. In general, the five boreholes contain fairly well-preserved, diverse microfossil faunas and floras consisting mainly of foraminifera, radiolaria, ostracods and diatoms. The studied interval was subdivided into three foraminiferid biostratigraphic intervals, in ascending stratigraphic order, the Stensioeina beccariiformis, Praeglobobulimina ovata and Pseudohastigerina wilcoxensis intervals and five biostratigraphic intervals based on additional microfossil groups (the Thalassiosiropsis wittiana, Fenestrella antiqua-Coscinodiscus morsianus, Ostracod, Aulacodiscus hirtus and Cenodiscus-Cenosphaera intervals). The intervals are more easily recognised in the two basinal boreholes, Ikermiut-1 and Kangamiut-1, than in the three more nearshore boreholes, Nukik-1, Nukik-2 and Hellefisk-1, due to a higher microfossil diversity and abundance.