The formaldehyde release from three different ground root canal sealer materials was examined. Ten specimens each of AH26, Amubarut, and N2 were stored under dry conditions for 6 months. An amount of ∼100 to 200 mg ground material was obtained from each sample by using a round bur and stored for 10 min in distilled water. The formaldehyde concentration of the immersion water was determined by high-performance liquid chromatography. The mean formaldehyde release per mg material was 6.6 (±2.5) μg for AH26 and 8.3 (±1.0) μg for Amubarut. A lower formaldehyde release was detectable by our method from the N2 samples (0.3 ± 0.1 μg/g; p < 0.0001). In conclusion formaldehyde release from ground root canal material is low, although a risk of an allergic reaction in susceptible patients cannot be excluded.