To measure the denaturation temperature (T d ) of demineralised dentine matrix as a function of infiltration with water vs. polar solvents vs. adhesive resins.Small discs of normal dentine were completely demineralised in 0.5M EDTA. Dried demineralised specimens were placed in water, methanol, ethanol, acetone, η-butanol or HEMA. Additional specimens were infiltrated with Prime&Bond NT and polymerised. All specimens sealed in high-pressure pans and scanned using differential scanning calorimetry (DSC).Demineralised dentine saturated with water showed a T d of 65.6°C that increased with saturation by methanol, ethanol, acetone, η-butanol or HEMA to 148.5°C. These increases in T d were inversely related to the molar concentration of the solvents and to their Hoy's solubility parameter for hydrogen bonding (δ h , p<0.01), as well as directly related to the cube root of their molecular weights (p<0.001). The presence of adhesive resins also increased the T d of demineralised matrices to even higher values depending if the resin bonded dentine was measured after 24h of water storage (166.8°C) or dry (172.7°C) storage.Solvents and monomers with low δ h values (i.e., 100% HEMA) increase the T d of demineralised dentine above that produced by solvents with higher δ h values such as methanol and water.