Plant regeneration was achieved in leaf callus derived from 15-day-old in vitro grown seedling and mature tree ofTrema orientalis naturally growing on chromite minewastes in a monsoon climate at Sukinda, India. Both types of explants formed callus which developed shoot buds in MS medium with benzyladenine (BA), 1-naphthaleneacetic acid (NAA) and adenine sulphate (Ads). Transfer of the 4-week-old calluses to MS basal medium supplemented with 11.11 μM (2.5 mg/l) BA and 1.34 μM (0.25 mg/l) NAA resulted in the highest shoot regeneration rate (82.1%) in leaf derived calluses developed from seedling source; the rate of shoot bud regeneration could be enhanced on incubation of the cultures under the light intensity of 62.5 μE/m 2 /s. The calluses obtained from leaves of mature trees were less efficient than those derived from seedling sources for shoot bud regeneration. Histological observations confirmed regeneration of shoot buds from leaf derived callus of T. orientalis. Excised shoots were induced to form roots by transfer to 12 MS + 0.053 μM (0.01 mg/l) NAA or 0.049 μM (0.01 mg/l) indole-3-butyric acid (IBA). The rooted plantlets were established in soil.