The Sr5(PO4)3F (S-FAP) nanoparticles were synthesized by a chemical co-precipitation method, and the effects of mixing modes on the particle’s morphology were investigated. The X-ray diffraction (XRD) results combined with the fourier transform-infrared spectroscopy (FT-IR) indicated that the as-synthesized powders were pure phase hexagonal apatite structure. The scanning electron microscope (SEM) combined with transmission electron microscopy (TEM) revealed that the particle’s morphology tended to transform from nanorods to nearly spherical particles when the mixing modes changed from droplet mixing to quick mixing. The distinctions in the morphology with different mixing modes were briefly analyzed predominantly taking into account the nucleation and growth rate.