We report on the generation of Ni nanoparticles and the synthesis of magnetic nanoparticles films by 100femtosecond (fs) Ti:sapphire laser ablation in vacuum. The nanoparticles sizes have been studied by atomic force microscopy, showing a mean size of ≈40nm. Vibrating sample magnetometry analysis has been performed to characterize magnetic properties of the deposited nanoparticles layers. The hysteresys loops clearly indicate that the Ni nanoparticles film behaves as a system of isolated magnetic particles. Our results evidence the potentiality of fs laser ablation, in vacuum, for the generation of nanoparticles and the deposition of nanoparticles films of magnetic materials.