In the present study, the distributions of exogenously administered horseradish peroxidase (HRP) and colloidal carbon in gonadal tissues were observed in both male and female mice using light microscopy. HRP was injected intravenously and colloidal carbon was directly injected into the gonadal parenchyma. Thereafter, the gonads were obtained for histological examination. The results showed that staining of HRP and carbon was detected in the ovaries at a low level. In contrast, much staining was observed in the interstitial cells of the testes for a long period. This suggests that ovarian tissues are less active in the uptake of exogenous materials than testicular tissues in vivo. However, immunohistochemical examination using anti-macrophage antibodies revealed that the ovaries contained a large number of macrophages, as did the testes, under normal conditions. Therefore, the results indicate that resident macrophages in the ovaries exhibit weak endocytic activity of foreign materials in vivo.