Gold nanotubule membranes were prepared by using electroless deposition of gold in the pores and on the surfaces of polycarbonate track-etched membranes, and the membranes were modified with Cl − . An electrochemical method (i–t) was then developed for studying the transport properties of bovine serum albumin (BSA) in these nanotubule membranes. In phosphate buffer solution (pH 7.4), when an electric field (l V) was applied between the two sides of the membrane, the response current decreased with the adding of BSA solution. The decrement of response current was in a linear relationship with the concentration of BSA in the range of 1.50 × 10 −10 –1.35 × 10 −9 M (r = 0.9980), and the limit of detection was 9.46×10 −11 M (S/N = 3).