We herein report a novel Pt-based electrocatalyst for direct methanol fuel cells (DMFCs) using multi-walled carbon nanotubes (MWCNTs) supported manganese oxide and poly(3,4-ethylenedioxythiophene) (PEDOT) nanocomposite (MnO x –PEDOT–MWCNTs) as catalyst support for Pt nanoparticles. The prepared nanocomposites are characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and electrochemical tests. The results demonstrate that Pt nanoparticles are uniformly deposited on the surface of MnO x –PEDOT–MWCNTs, and their dispersion and electrochemical active surface area (ECSA) are obviously improved. The methanol electrooxidation activity and stability of the Pt/MnO x –PEDOT–MWCNTs are significantly enhanced as compared with the Pt/PEDOT–MWCNTs and Pt/MWCNTs catalysts. This study implies that the as-prepared Pt/MnO x –PEDOT–MWCNTs will be a promising candidate as an anode electrocatalyst in DMFCs.