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A new expression system for Lactococcus lactis based on the salt inducible BusA promoter and the BusR repressor gene of L. lactis MG1363 was developed. To achieve salt induction, the expression of BusR was modulated by introducing mutations to its promoter sequence. An activity of 6.0μkatl −1 of the model enzyme Lactobacillus amylovorus α-amylase was achieved in the bioreactor cultivation...
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