Adult stem cells are more appropriate for the practice of regenerative medicine than ES cells or iPS cells. Hydrogen sulfide (H 2 S) which works in several physiological processes increases hepatic differentiation from human deciduous tooth pulp stem cells (hDTPSC). The present study assessed differentiation of hDTPSC towards pancreatic lineages, and the effect of H 2 S on the differentiation. The cells were isolated from deciduous tooth pulp and were grown in DMEM supplemented with 10% FBS. CD117 + cells were magnetically separated, and were subjected to differentiation protocol. Expression of a panel of pancreatically related transcription factors and WNT/ beta catenin signaling pathway were determined with RT-PCR. Immunocytochemistry and flow cytometry of pancreatically related marker were carried out after the differentiation. Concentration of secreted insulin and c-peptide in the medium was determined with ELISA. During the differentiation the cultures were exposed to 0.1ng/mL air of hydrogen sulfide. The cells non-exposed to hydrogen sulfide were used as control. Insulin, glucagon, somatostatin, and pancreatic polypeptide were positive by immunofluorescence and flow-cytometry. H 2 S increased the expression of insulin. WNT signaling pathways were activated in the differentiation, and H 2 S enhance these pathways. The concentration of insulin and c-peptide in H 2 S sample increased compared with control.CD117 + fractions of hDTPSC are capable of differentiate toward pancreatic cells, and the differentiation of pancreatic cells was increased by hydrogen sulfide. Low concentration of H 2 S may enhance the differentiation property. Our method of pancreatic differentiation may have great potential for future cell therapy of pancreatic disorders.