CABANAC, M. AND F. LABERGE.Fever in goldfish is induced by pyrogens but not after handling. PHYSIOL BEHAV. 63(3) 377-379, 1998.-Six goldfish, Carassius auratus, weighing 2.5 to 4 g were placed individually in an aquarium with two communicating chambers. One chamber was thermostatted at 34°C, the other at 37°C. In control Session a, without external intervention, fish selected the cooler chamber most of the time and stayed only 4.8 ± 1.1 min/2 h at 37°C. In Session b, infectious fever was assayed: pyrogen (Salmonella typhosa LPS, or human interleukin-2) was injected intracranially and fish stayed 44.7 ± 15.3 min/2 h at 37°C. In Session c, behavioral stress was achieved by chasing the fish with a net, catching it, handling it out of water, and injecting 10 μL of saline intracranially. Fish stayed 2.7 ± 1.0 min/2 h at 37°C. Analysis of variance showed that stay at 37°C was significantly longer in Session b than a and c, and that Sessions a and c were not significantly different from one another. This result confirms the existence of behavioral fever, but does not support the hypothesis of fever in fish after handling.