We study Higgs boson production and decay in a certain class of little Higgs models with T-parity in which some T-parity partners of the Standard Model (SM) fermions gain their masses through Yukawa-type couplings. We find that the Higgs boson production cross section of a 120 GeV Higgs boson at the CERN LHC via gg fusion process at one-loop level could be reduced by about 45%, 35% and 20%, as compared to its SM prediction, for a relatively low new particle mass scale f=600, 700 and 1000 GeV, respectively. On the other hand, the weak boson fusion cross section is close to the SM value. Furthermore, the Higgs boson decay branching ratio into di-photon mode can be enhanced by about 35% in small Higgs mass region in certain case, for the total decay width of Higgs boson in the little Higgs model is always smaller than that in the SM.