The interaction of a model strained metal film with oxygen is studied by scanning tunneling microscopy. Two monolayers of copper on Ru(0001) present a well-defined dislocation network composed of threading dislocations and Shockley partial dislocations separating areas of fcc and hcp stacking. We find that oxygen first reacts with these threading dislocations. Then, for exposures up to ~0.4L O 2 , new threading dislocation arrays appear on the surface. With the addition of more oxygen, the mesoscopic structure of the film changes from a striped array of Shockley partials to a disordered array of triangular fcc regions bounded by dislocations, as oxygen proceeds to etch away the hcp areas of the copper film.