The corresponding 1,2,3-triazole derivatives were readily obtained when activated acetylenic fatty acid esters, viz. methyl 2-undecynoate (1), 14-oxo-12-octadecynoates (3), 12-oxo-10-octadecynoate (5), 8-oxo-11(E/Z)-octadecen-9-ynoate (7), 8-oxo-11,12-epoxy-9-octadecynoate (9), were reacted with sodium azide in dimethylformamide. An α-epoxy group to the acetylenic function was also found to be activating the triazolation process. Thus, methyl 11,12-epoxy-9-octadecynoate (11) reacted with sodium azide under reflux in ethanol in the presence of ammonium chloride to give a regiospecific derivative, namely 4-(1-azido-2-hydroxy-octyl)-5-(7-carbomethoxyheptyl)-1,2,3-triazole (12). The structure of the various derivatives were characterized by a combination of infrared, nuclear magnetic resonance spectroscopic and mass spectrometric analyses.