Amongst 25 extra-intestinal clinical isolates of Clostridium difficile, A + B + (72%) and A − B + (4%) toxigenic phenotypes, as well as the non-toxigenic phenotype (A − B − ) (24%), were identified. The A − B − isolates did not express toxin, yet carried part of the tcdA and tcdB gene and are of a previously unreported toxinotype. Six A + B + isolates also carried binary toxin genes. Resistance to erythromycin (20%), clindamycin (48%), tetracycline (16%), moxifloxacin (16%) and imipenem (11%) occurred but with no apparent correlation to phenotype. None of the strains was resistant to vancomycin or metronidazole. Imipenem-resistance decreased by EDTA, but susceptibility to meropenem suggests the presence of an imipenem specific metalloenzyme.