Three different chromium-resistant microorganisms (Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.) were tested with regard to their EPS production at different pH levels, temperatures, Cr(VI) concentrations, and incubation periods. The optimum pH level was 7 for P. aeruginosa and Micrococcus sp., while it was 8 for Ochrobactrum sp. according to the highest EPS amount at 100mg/L Cr(VI) concentration. The highest production of EPSs by the three bacteria was obtained under different environmental conditions. P. aeruginosa produced the highest EPS (863.3mg/L) after incubation for 96h on media with 50mg/L Cr(VI) at 20°C, Micrococcus sp. gave the highest yield (444.6mg/L) after incubation for 72h on media with 100mg/L Cr(VI) at the same temperature, and Ochrobactrum sp. had the highest production (430.5mg/L) on media with 150mg/L Cr(VI) at 30°C at the end of 48h of incubation.