Twenty-eight tetranucleotide STR markers were analysed from a point of view of their applicability in forensic genetic practise. Polymorphism, discrimination and paternity exclusion indices for particular loci were compared based on allele distribution in central Poland population. The highest heterozygosity of 0.945 and polymorphism information content of 0.950 were determined for the SE33 locus. The 33 different alleles, with frequency below 0.1, were revealed in relevant population. With the highest power of discrimination of 0.991, and power of exclusion of 0.888, locus SE33 is the most useful marker in forensic identification as well as in kinship analysis. After SE33, the subsequent most informative markers, out of the 28 investigated in central Poland population, are D2S1338 and D12S391, which reach the power of discrimination of 0.972 and power of exclusion of 0.761.