The photoinduced interaction of fluorescein ester derivatives, fluorescein's butyl ester (FL4) and fluorescein's anthraquinone-methyl ester (FL-AQ), and colloidal CdS was examined by absorption, fluorescence spectroscopy, and photoinduced ESR spectroscopy. It is found that FL4 and FL-AQ molecules are adsorbed on the surfaces of CdS colloid by an electrostatic interaction forming the surface complex of the type CdS-FL4 or CdS-FL-AQ. The apparent association constant (K a p p ) and the degree of association (α) of CdS-FL4 obtained from absorption spectra are 2.25x10 4 M - 1 and 0.78, respectively. The values of K a p p and α of CdS-FL4 as determined from fluorescence spectra are 1.54x10 4 M - 1 and 0.82, respectively, which matches well with that determined from the absorption spectra changes. And the values of K a p p and α of CdS-FL-AQ obtained from absorption spectra are 4.18x10 4 M - 1 and 0.83, respectively. These data indicate that there is a strong interaction between the dye and the CdS particle surface. But there was no evidence for interfacial electron transfer from FL4 or FL-AQ to colloidal CdS by photoinduced ESR experiments. The fluorescence quenching is due to the formation of a nonfluorescent complex. The related phenomena are discussed in this paper.