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Chemical and enzymatic synthesis of glycoconjugates 5: one-pot regioselective synthesis of bioactive galactobiosides using a clonezyme TM thermophilic glycosidase library
Enzymatic synthesis of galactobiosides using a versatile CLONEZYME TM thermostable glycosidase library was studied. One-pot transglycosylation reactions were demonstrated to synthesize β(1->4), β(1->6), and α(1->6) disaccharide sequences with high regioselectivity and moderate to high yields.