Properties of film from cuttlefish (Sepia pharaonis) ventral skin gelatin without and with partial hydrolysis (1.2% degree of hydrolysis), as influenced by H 2 O 2 and Fenton’s reagent at different levels, were investigated. Films treated with H 2 O 2 (0.01–0.04M) and Fenton’s reagent [H 2 O 2 (0.01–0.04M)+FeSO 4 (0.001–0.004M)] had higher tensile strengths (TS) but similar or lower elongations at break (EAB), compared with the control film (p<0.05). Slight differences in water vapour permeability (WVP) were observed for all films. Films treated with Fenton’s reagent had a lower L ∗ -value but higher a ∗ -, b ∗ - and ΔE ∗ -values, while films treated with H 2 O 2 had lower b ∗ -values (p<0.05), than had the control film. Cross-linking was pronounced in films treated with H 2 O 2 or Fenton’s reagent and was associated with increased heat stability. Films treated with Fenton’s reagent had the lowest solubility in water (p<0.05). However, fragmentation more likely took place when Fenton’s reagent (at a higher level) was used. Generally, similar results were noticeable between films from gelatin with and without partial hydrolysis. Thus, H 2 O 2 and Fenton’s reagent directly affected the properties of film from cuttlefish skin gelatin, regardless of hydrolysis.