This study was designed to analyze whether Mental Rotation (MR) played a role as a mediating variable for sex-related differences in Visualization (VZ). Two psychometric tests measuring MR and VZ were applied to a representative sample of 309 males and 390 females in their last year of high school. Three non-zero correlations between sex and MR, sex and VZ, and MR and VZ were found, and the effect of sex on VZ was eliminated when MR was introduced as a covariable. When three subgroups of different VZ ability were made by dividing up the VZ distribution by the first and third quartiles, sex-differences were only found for the high-scorers group, for which previous results were replicated. Results clearly indicate that MR is a plausible mediator variable for sex differences in VZ when such differences do exist. Theoretical, methodological and practical consequences of these results are discussed.