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A DNA-based piezoelectric biosensor has been here studied in terms of probe immobilisation and DNA sample pre-treatment. The biosensor is specific for the detection of the mecA gene of methicillin-resistant Staphylococcus aureus (MRSA).Methicillin-resistant S. aureus is responsible of several infections in humans, like pneumonia, meningitis and endocarditic. MRSA is also a major cause of hospital-acquired...
A sequential injection analysis (SIA) methodology for the fluorimetric determination of aminocaproic acid in pharmaceutical formulations is proposed. The developed analytical procedure is based on the derivatisation reaction of the aminocaproic primary amine with o-phthalaldehyde (OPA) and N-acetylcysteine (NAC) and fluorimetric detection of the formed product (λ ex =350nm; λ em =450nm)...
The determination of metals in lubricating oil has been used as an important way to prevent components failures, to provide environmental information and in some cases, to identify adulteration. In this work, an effective and simple procedure is proposed for Cu and Pb determination in lubricating oils. An ultrasonic bath was employed for extraction of these elements from oil samples in a mixture 1:1...
Microchip electrophoresis is a promising technique for analysis of bio-molecules. It has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples. Plastic chip has the potential of mass production for clinical use for its advantages in biocompatibility and low cost. In this work, the method for fabrication of poly(methyl methacrylate) (PMMA) chip was described, and...
A new kind of near-infrared fluorescence agent, tricarbochlorocyanine dye (Cy.7.Cl), had been synthesized in house and used for near-infrared spectrofluorimetric determination of hydrogen peroxide (H 2 O 2 ) by flow injection analysis (FIA) for the first time. The oxidation reaction of Cy.7.Cl with H 2 O 2 occurred under the catalysis of horseradish peroxidase (HRP)...
Cytochrome c (Cyt. c) was immobilized on the 11-mercaptohendecanoic acid (MUA)-modified gold electrode. The electrode was stable and sensitive to Cyt. c. Later, DNA was also immobilized on the two-layer modified electrode. Cyclic voltammetry studies show that Cyt. c can interact with dsDNA and ssDNA. The binding site sizes were determined to be 15 base pairs per Cyt. c molecule with dsDNA and 30 nucleotides...
The construction of the favorable initial iterative vectors is the key to iterative target transformation factor analysis (ITTFA). A tentative approach to construct the better initial vectors, which is based on the chromatographic information provided by evolving factor analysis (EFA), is proposed. A region, which contains the peak position at maximum height, is determined. Several elements in the...
This paper introduces a simple, fast and reliable electroanalytical method for differential-pulse polarography based on electrochemical reduction at a dropping mercury electrode. The method was validated for the determination of 2-ethylhexyl-4-methoxycinnamate (EHMC) alone and in association with 4-methylbenzylidene camphor (MBC) or 2-hydroxy-4-methoxybenzophenone (BENZ-3) in samples of commercial...
A novel simple, sensitive and rapid kinetic-spectrophotometric method is proposed for the determination of trace amounts of bromide. The method is based on its catalytic effect on the oxidation of methylene blue (MB) by hydrogen peroxide in strongly acidic solution. The oxidation reaction is activated by large amounts of chloride and can be monitored spectrophotometrically by measuring the decrease...
Ultra Performance Liquid Chromatography (UPLC) is a relatively new technique giving new possibilities in liquid chromatography, especially concerning decrease of time and solvent consumption. UPLC chromatographic system is designed in a special way to withstand high system back-pressures. Special analytical columns UPLC Acquity UPLC BEH C 18 packed with 1.7μm particles are used in connection...
A simple DNA diagnosis method using microfluidics has been developed which requires simple and straightforward procedures such as injection of sample and probe DNA solutions. This method takes advantage of the highly accurate control of fluids in microchannels, and is superior to DNA microarray diagnosis methods due to its simplicity, highly quantitative determination, and high-sensitivity. The method...
A reagent tablet for determination of fluoride ion has been prepared using ethylenediamine-N,N,N′,N′-tetraacetate complex of zirconium (Zr-EDTA), 3-hydroxy-2′-flavone (FS) and an appropriate pH buffer. Dissolving of the tablet into water exhibits an intense blue fluorescence (λ max =460nm) upon excitation at 377nm and the fluorescence intensity decreases with the presence of fluoride ion....
A green chromatographic analytical method for determination of Tartrazine, Brilliant Blue and Sunset Yellow in food samples is proposed. The method is based on the modification of a C18 column with a 0.25% (v/v) Triton X-100 aqueous solution at pH 7 and in the usage of the same surfactant solution as mobile phase without the presence of any organic solvent modifier. After the separation process on...
The application of a multi-frequency laser source for the use in matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described. An elliptically polarized beam of a Nd:YAG laser emitting at 355nm (200mJ) is focused into a Raman shifter, filled with high pressure hydrogen. As a result, numerous Raman lasers (including vibrational and rotational Raman emissions...
4-Aminobenzoic acid (4-ABA) was covalently grafted on a glassy carbon electrode (GCE) by amine cation radical formation during the electrooxidation process in 0.1M KCl aqueous solution. X-ray photoelectron spectroscopy (XPS) measurement proves the presence of 4-carboxylphenylamine on the GCE. Electron transfer processes of Fe(CN) 63− in solutions of various pHs at the modified electrode...
The determination of uranium is important for environmental radioactivity monitoring, which investigates the releases of uranium from nuclear facilities and of naturally occurring radioactive materials by the coal, oil, natural gas, mineral, ore refining and phosphate fertilizer industries, and it is also important for studies on the biogeochemical behavior of uranium in the environment. In this paper,...
Due to new findings, the methodology based on room-temperature ultrasonic irradiation (sonolysis) for conversion of organomercurials into inorganic mercury [J.L. Capelo, I. Lavilla, C. Bendicho, Anal. Chem. 72 (2000) 4979–4984.] is further investigated. Inorganic mercury is selectively determined by Flow Injection-Cold Vapour Atomic Absorption Spectrometry (FI-CV-AAS) using SnCl 2 /HCl. Complete...
A flow injection spectrophotometric method has been developed for the determination of dissolved and total amounts of iron in tap and natural water samples. The method for the determination of iron employs a sample acidification step in order to decompose iron hydroxide and iron-complexes into free iron, Fe(III) and Fe(II). The amounts of free iron were detected using a catalytic action of Fe(III)...
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