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A glucose sensitive enzymatic thermal sensor based on a pair of commercial microthermal probes was fabricated by cross-linking of the corresponding enzymes (glucose oxidase and catalase) with bovine serum albumin onto the chip surfaces. The characteristics of this sensor were optimised by testing it in different conditions. To find the maximal sensitivity, the calibration curves of this sensor were...
Ferric 2-hydroxy-1-naphthaldehyde thiosemicarbazone (Fe III -HNT) has been synthesized and used to mimic the active group of horseradish peroxidase (HRP). The catalytic characteristics of this mimic-enzyme catalyst in the oxidation reaction of ascorbic acid with hydrogen peroxide has been studied. The experimental results showed that this peroxidase model compound has similar catalytic...
A new amperometric glucose sensor based on the glucose oxidase immobilized on pyrolytic graphite (PG) modified with tetraammineplatinum(II) chloride (TAPtCl) and 5,10,15,20-tetrakis (4-methoxy-phenyl)-21H,23H-porphine cobalt(II) (TMPPCo) as well as Nafion was studied. The performances amongst the glucose sensors with or without TAPtCl or/and TMPPCo measured with oxygen present in the solution were...
A novel chemiluminescence (CL) system was evaluated for the determination of hydrogen peroxide, glucose and ascorbic acid based on hydrogen peroxide, which has a catalytic-cooxidative effect on the oxidation of luminol by KIO 4 . Hydrogen peroxide can be directly determined by luminol-KIO 4 -H 2 O 2 CL system. The detection limit was 3.0x10 -8 mol l ...
Electropolymerized film of Meldola's blue (MB) was prepared and demonstrated as electron shuttle between the immobilized horse peroxidase (HRP) and glassy carbon electrode (GCE) for sensing hydrogen peroxide (H 2 O 2 ) produced by enzyme catalytical reactions. Electrochemical polymerization of Meldola's blue was carried out by cyclic voltammetry (CV) in a phosphate buffer solution...
A chemiluminescence (CL) biosensor on a chip coupled to microfluidic system is described in this paper. The CL biosensor measured 25x45x5 mm in dimension, was readily produced in analytical laboratory. Glucose oxidase (GOD) was immobilized onto controlled-pore glass (CPG) via glutaraldehyde activation and packed into a reservoir. The analytical reagents, including luminol and ferricyanide, were electrostatically...
A rapid method for the determination of Pb, Cd, Cu, Cr, Co, Ni, Mn and Zn in honey and sugars without prior digestion or ashing of the sample was developed, using inductively coupled plasma atomic emission spectrometry (ICP-AES). The critical instrumental parameters such as sample flow rate and radio frequency incident power were thoroughly optimized. The effect of matrix type and its concentration...
Electrochemical glucose enzyme biosensors have been prepared on carbon film electrodes made from carbon film electrical resistors. Evaluation and characterisation of these electrodes in phosphate buffer saline solution has been carried out with and without pretreatment by cycling in perchloric acid or at fixed applied potential. Both pretreatments led to a reduction in the carbon surface oxidation...
This paper describes glucose nanosensors based on the co-electrodeposition of a poly(vinylimidazole) complex of [Os(bpy) 2 Cl] +/2+ and glucose oxidase (GOD) on a low-noise carbon fiber nanoelectrodes (CFNE). The SEM image shows that the osmium redox polymer/enzyme composite film is uniform. The film modified CFNE exhibits the classical features of a kinetically fast redox couple bound...
A highly selective, fast and stable biosensor for determination of glucose in soluble coffee has been developed. The biosensor electrode consist of a thin film of ferric hexacyanoferrate (Prussian Blue or PB) electrodeposited on the glassy carbon electrode (GCE) (to provide a catalytic surface for the detection of hydrogen peroxide) glucose oxidase immobilized on top of the electrode and a Nafion...
An amperometric glucose ring-disk biosensor based on a ruthenium complex mediator of low redox potential was fabricated and evaluated. This thin-layer radial flow microsensor (10μl) with ring-disk working electrode displayed remarkable amperometric sensitivity. For Ru 3 (μ 3 -O)(AcO) 6 (Py) 3 (ClO 4 ) (Ru-Py), a trinuclear oxo-acetate bridged cluster, a reversible...
This work reports a hybrid flow-injection analysis (FIA)/sequential-injection analysis (SIA) method for the rapid enzymatic assay of glucose with soluble glucose oxidase (GOD). The method relies on the sequential injection of segments of the sample and of a solution of enzyme by means of a multi-port selection valve in a flowing water stream. As the two zones are swept downstream, they overlap and...
The preparation and characterization of an amperometric glucose biosensor based on the entrapment of glucose oxidase (GOx) in a polyacrylamide microgel is described. This study proves that polyacrylamide microgels provide an excellent matrix for GOx immobilization that can be used as a biological material in amperometric biosensors. The interference produced by ascorbic and uric acid has been eliminated...
An experiment was developed as a simple alternative to existing analytical methods for the simultaneous quantitation of glucose (substrate) and glucuronic acid (main product) in the bioprocesses Kombucha by using FTIR spectroscopy coupled to multivariate calibration (partial least-squares, PLS-1 and artificial neural networks, ANNs). Wavelength selection through a novel ranked regions genetic algorithm...
Despite wide application of cellulose-azure as a substrate for measuring cellulase activity, there is no quantification of hydrolysis rate or enzymatic activities using this substrate. The aim of this study was to quantify the hydrolysis rate in terms of product formation and dye released using cellulose-azure. The amount of dye released was correlated with the production of glucose and the enzyme...
A novel copper incorporated self-assembled monolayers (SAMs) modified gold electrode (Cu/SAMs) for determination of glucose was developed by electrodepositing Cu particles on the SAMs of hexanethiol. The scanning electron microscopic (SEM) images showed that copper formed orbicular particles of nanosizes on the SAMs, which was much different from the fractal-like particles of copper formed at gold...
A long-life capillary enzyme bioreactor was developed that determines glucose concentrations with high sensitivity and better stability than previous systems. The bioreactor was constructed by immobilizing glucose oxidase (GOx) onto the inner surface of a 0.53mm i.d. fused-silica capillary that was part of a continuous-flow system. In the presence of oxygen, GOx converts glucose to gluconic acid and...
An electrochemical sensing platform was developed based on the magnetic loading of carbon nanotube (CNT)/nano-Fe 3 O 4 composite on electrodes. To demonstrate the concept, nano-Fe 3 O 4 was deposited by the chemical coprecipitation of Fe 2+ and Fe 3+ in the presence of CNTs in an alkaline solution. The resulting magnetic nanocomposite brings new capabilities...
This paper reports a flow-injection analysis (FIA) of glucose not using enzyme based on the electrocatalytic oxidation of glucose at a nickel electrode. The electrocatalytic mechanism and quantificational method of glucose have been investigated. The current intensity of the electrocatalytic oxidation to glucose at the potential of 550mV is proportional to the concentration of glucose over the range...
A novel voltammetric method for the determination of β-d-glucose (GO) is proposed based on the reduction of Cu(II) ion in Cu(II)(NH 3 ) 42+ –GO complex at lanthanum(III) hydroxide nanowires (LNWs) modified carbon paste electrode (LNWs/CPE). In 0.1molL −1 NH 3 ·H 2 O–NH 4 Cl (pH 9.8) buffer containing 5.0×10 −5 molL −1 Cu(II) ion,...
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