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A flow-injection system with a co-immobilized malate dehydrogenase/reduced nicotineamide adenine dinucleotide (NADH) oxidase reactor and a chemiluminometer is described for the determination of freel -malate in wine. Malate dehydrogenase and NADH oxidase were co-immobilized on poly(vinyl alcohol) beads and packed into a stainless-steel column (5 cm 4 mm i.d.). The hydrogen peroxide produced was...
A novel flow sensor based on chemiluminescence (CL) for the determination of ascorbic acid has been proposed. The analytical reagents, luminol and ferricyanide, were both immobilized on an anion-exchange resin column. The CL signal produced by the reaction between luminol and ferricyanide, which were eluted from the column through sodium phosphate injection, was decreased in the presence of ascorbic...
A luminol chemiluminescence (CL) method was developed for the determination of glutathione (GSH). GSH was indirectly determined by measuring the amount of hydrogen peroxide formed during the Cu(II)-catalysed oxidation of GSH with oxygen. The amount of hydrogen peroxide formed was continuously measured using the Arthromyces ramosus peroxidase-catalysed luminol CL reaction. The CL intensities at...
A flow-injection method for the determination of glycerol in wine is described. Glycerol dehydrogenase and NADH oxidase were co-immobilized on poly (vinyl alcohol) beads and incorporated in a flow-injection system. The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate (III) reaction. Wine was diluted 1000-fold with water and sample solution (50 μl) was...
Combinations of 4-substituted phenylboronic acids [phenyl, iodo, bromo, and trans-4-(3-propenoic acid) substituents] have been discovered to have synergistic effects in the horseradish peroxidase (HRP) catalyzed chemiluminescent oxidation of luminol. Three types of effect have been observed: 1. synergistic reduction in the background light emission of a luminol-peroxide assay reagent to a value lower...
A flow-injection analytical method for the determination of hydrochlorothiazide is presented. The method is based on the chemiluminescence reaction of hydrochlorothiazide with cerium(IV) in sulphuric acid, sensitized by the fluorescent dye rhodamine 6G. The proposed procedure allows quantitation of hydrochlorothiazide in the concentration range of 0.33–130 μmol l −1 with a detection limit...
A chemiluminescence method for the determination of citric acid was developed. The method is based on the enhancement of citric acid on the chemiluminescence light emission of tris-(2,2'-bipyridine)ruthenium(II). In the presence of tris-(2,2'-bipyridine)ruthenium(II), upon the addition of Ce(IV), resulted in intense light emission. The emission intensity is greatly enhanced by the presence of citric...
A chemiluminescent method using flow injection is described for the determination of cefadroxil monohydrate. The method is based on the chemiluminescence reaction of cefadroxil with potassium permanganate in sulphuric acid, sensitized by quinine. The proposed procedure allows the determination of cefadroxil over the concentration range 0.1-30 μg ml -1 with a detection limit of 0.05 μg...
A novel chemiluminescence (CL) system for ammonium ion combined with flow-injection analysis is presented in this paper. It is based on the CL reaction between luminol, immobilized electrostatically on an anion-exchange column, and chlorine electrochemically generated on-line via a platinum electrode from hydrochloric acid in a coulometric cell. Ammonium ion reacts with chlorine and decreases the...
A novel chemiluminescence (CL) system was evaluated for the determination of hydrogen peroxide, glucose and ascorbic acid based on hydrogen peroxide, which has a catalytic-cooxidative effect on the oxidation of luminol by KIO 4 . Hydrogen peroxide can be directly determined by luminol-KIO 4 -H 2 O 2 CL system. The detection limit was 3.0x10 -8 mol l ...
A chemiluminescence (CL) detection for the determination of sulfite using the reaction of Ru(bipy) 32+ (bipy=2,2′-bipyridyl) -SO 32− -KMnO 4 is described. The concentration of sulfite is proportional to the CL intensity from 5.0×10 −8 to 1.25×10 −4 mol l −1 . The limit of detection is 2.5×10 −8 mol l −1 and the relative standard...
An accurate selective flow injection chemiluminescence (CL) method for the assay of perphenazine was explored. In the method 394 ppm permanganate solution was used as a chemiluminogenic reagent in 0.289 mol dm -3 sulphuric acid media. A photomultiplier tube was used as a detector at a total flow rate of 4.94 ml/min. Perphenazine was determined by a linear calibration plot of the following...
A flow-injection analysis (FIA) for the determination of dopamine has been developed. The method is based on the inhibition effect of dopamine on the iron(II)-induced chemiluminescence (CL) of 10,10'-dimethyl-9,9'-biacridinium dinitrate (lucigenin). The presence of a non-ionic surfactant, polyoxyethylene (23) lauryl ether (Brij 35), caused an increase in the inhibition effect. The present method allows...
An automated flow-injection system combining a pyruvate oxidase reaction and chemiluminescence for the detection of phosphate ion in river water has been developed. In this research, we used pyruvate oxidase G (PyrOxG), from Aerococcus viridans, immobilizing it on N-hydroxysuccinicacidimido beads without a cross-linker. In this sensor system, which was constructed as a trial system of desktop type,...
A novel chemiluminescence (CL) flow system for the determination of quinine is described. It is based on the direct chemiluminescence reaction of quinine and cobalt(III) in sulfuric acid medium. The unstable Co(III) was on-line electrogenerated by constant-current electrolysis. The chemiluminescence intensity was linear with a quinine concentration in the range of 0.1-100 μg ml -1 . The...
A highly rapid chemiluminescent assay for the determination of superoxide dismutase (SOD) activity in erythrocytes was developed. The inhibition of the luminescent emission caused by the decrease of generated superoxide anions was measured. The aim of this work was to verify the application of a non amplified luminol SOD luminescent assay (CLM) in erythrocytes starting from an amplified method already...
A flow injection method with chemiluminescence (CL) detection is proposed for the determination of sodium 2-mercaptoethane sulfonate (MESNA), a drug often used to reduce the urotoxic effects of antineoplastic alkylating agents. The procedure is based on the reaction of the thiol with Ce(IV) in a sulfuric acid medium and measurement of the CL intensity produced by quinine used as a sensitizer. The...
Local anaesthetics procaine hydrochloride (I), benzocaine (II), and tetracaine hydrochloride (III) were determined by the technique of sequential injection analysis (SIA) with chemiluminescence (CL) detection. The CL was emitted during the oxidation of the analytes by permanganate in aqueous sulphuric acid in the presence of various CL enhancers (4-hydroxybiphenyl, Rhodamine B, glycolaldehyde, glutaraldehyde...
A fast and sensitive chemiluminescent (CL) enzyme immunoassay for clenbuterol (CLB) analysis in bovine urine has been developed. Clenbuterol (CLB) specific polyclonal antibodies were raised in rabbit using a CLB azo derivative conjugated with ovalbumin. Horseradish peroxidase (HRP) was used as label and conjugated with the same derivative. In the developed competitive method, antibodies were immobilized...
A novel chemiluminescence (CL) system, cobalt(II)-hydrogen peroxide-hydrogencarbonate without luminescent reagents, was described. When cobalt(II) was injected into mixed solution of 0.4 mol l -1 hydrogencarbonate and 0.01 mol l -1 hydrogen peroxide in a flow injection system, CL occurred, which was significantly enhanced when rhodamine B coexisted. The CL emission intensity...
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