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In this work, a fluorescence turn-on method for copper(II) detection is reported. A molecular beacon (MB) was designed as a template. Cu 2+ was reduced to Cu + in the presence of a reductant (ascorbic acid). Two short single-stranded oligonucleotides one was labeled with a 5′-alkyne and the other with 3′-azide group, proceeded a template-dependent chemical ligation through the Cu(I)-catalyzed...
DNA is an excellent molecular scaffold for the preparation of copper nanoparticles (CuNPs). However, screening and designing an efficient template for the formation of fluorescent CuNPs remains a challenge. Herein, we synthesized CuNPs using a novel hairpin DNA template with a poly T loop and a random double strand stem which performs efficient in the formation of fluorescent CuNPs. Besides, the additive...
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