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The effect of axial temperature gradient (ATG) along a microcolumn on the separation performance at both isocratic and gradient elution mode was investigated. A thermostat system was designed to form an ATG along the packed column. Polycyclic aromatic hydrocarbons (PAHs) were separated on a 0.53mm ×150mm i.d. 5μm C 18 microcolumn, with water and acetonitrile as mobile phase. The separation...
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