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A mediator-free amperometric biosensor for screening organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system based on anticholinesterase activity of OPs to immobilized acetylcholinesterase enzyme (AChE) has been developed. The enzyme biosensor is prepared by entrapping AChE in Al 2 O 3 sol–gel matrix screen-printed on an integrated 3-electrode plastic chip. This strategy...
A flow-injection system for the quantification of pyruvate based on the coupled reactions of pyruvate decarboxylase (PDC) and aldehyde dehydrogenase (AlDH) was conceived and optimized. A co-immobilized PDC and AlDH reactor was introduced into the flow line. Sample and reagent (NAD + ) were injected into the flow line by an open sandwich method and the increase of NADH produced by the immobilized-enzyme...
This paper reports on a microfluidic device for the flow-injection/electrochemistry analysis of nitroaromatic explosive. The response is very fast (150assays/h), highly sensitive (detection limit 7.0μgL −1 ), reproducible and stable (R.S.D.=2.0%; n=30) and linear (over 20–100μgL −1 range). Relevant experimental parameters have been optimized. The new microsystem offers great promise...
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