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A long-life capillary enzyme bioreactor was developed that determines glucose concentrations with high sensitivity and better stability than previous systems. The bioreactor was constructed by immobilizing glucose oxidase (GOx) onto the inner surface of a 0.53mm i.d. fused-silica capillary that was part of a continuous-flow system. In the presence of oxygen, GOx converts glucose to gluconic acid and...
An electrochemical sensing platform was developed based on the magnetic loading of carbon nanotube (CNT)/nano-Fe 3 O 4 composite on electrodes. To demonstrate the concept, nano-Fe 3 O 4 was deposited by the chemical coprecipitation of Fe 2+ and Fe 3+ in the presence of CNTs in an alkaline solution. The resulting magnetic nanocomposite brings new capabilities...
This paper reports a flow-injection analysis (FIA) of glucose not using enzyme based on the electrocatalytic oxidation of glucose at a nickel electrode. The electrocatalytic mechanism and quantificational method of glucose have been investigated. The current intensity of the electrocatalytic oxidation to glucose at the potential of 550mV is proportional to the concentration of glucose over the range...
A novel voltammetric method for the determination of β-d-glucose (GO) is proposed based on the reduction of Cu(II) ion in Cu(II)(NH 3 ) 42+ –GO complex at lanthanum(III) hydroxide nanowires (LNWs) modified carbon paste electrode (LNWs/CPE). In 0.1molL −1 NH 3 ·H 2 O–NH 4 Cl (pH 9.8) buffer containing 5.0×10 −5 molL −1 Cu(II) ion,...
An amperometric biosensor for sensitive and selective detection of glucose has been constructed by using highly dispersed Pt nanoparticles supported on carbon nanotubes (Pt-MWCNTs) as sensing interface. The Pt-MWCNTs were synthesized by using the two-step pyrolysis method. This composite shows good electrocatalytic activity towards the oxidation of glucose in alkaline and thus can be used to selectively...
Binding of wheat germ agglutinin (WGA) on glucosamine-modified magnetic microbeads was investigated with voltammetry. A magnetic bead was considered as a cell, and the beads with amino groups were modified with the sugar by using a cross-linking reagent. To evaluate the binding, glucose labeled with an electroactive daunomycin was prepared as a probe. After WGA and the beads were mixed in 0.1M phosphate...
In this paper, the formation of gold nanoparticles (Au NPs) as a result of the thermo-active redox reaction of chlorauric acid (HAuCl 4 ) and glucose in alkaline medium was identified by measuring the plasmon resonance absorption, localized surface plasmon resonance (LSPR), and transmission electron microscopy (TEM) images, for the formation of Au NPs displays characteristic plasmon resonance...
In this paper, the electrochemiluminescence (ECL) behavior of bis-[3,4,6-trichloro-2-(pentyloxycarbonyl)-phenyl] oxalate (BTPPO) at glassy carbon electrode (GCE) in phosphate buffer solution in the presence of hydrogen peroxide has been investigated when linear sweep voltammetry was applied. The optimum chemical conditions and electrochemical parameters for this ECL system have been investigated in...
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