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A sensitive sequential injection analysis (SIA) methodology for the fluorimetric determination of naproxen is proposed. The developed automatic analytical procedure is based on the complexation of naproxen with β-cyclodextrin (β-CD) yielding an enhanced fluorimetric signal (λ ex =280nm, λ em =356nm).Linear calibration plots were obtained for naproxen concentrations up to 1×10 −5...
We developed new ELISA techniques in sequential injection analysis (SIA) mode using microreactors with content of a few microliters. We immobilized antibodies on magnetic beads 1.0μm in diameter, injected the beads into microreactors and applied rotating magnetic fields of several hundred gauss. Magnetic beads, suspended in liquid in density of approximately 10 9 –10 10 particles per...
A novel autoadaptative sequential injection system for the analysis of nitrite is described. The automatic determination uses a direct spectrophotometric method, based on the Griess–Ilosvay reaction. In this method the absorbance of the purple azo dye formed is measured at 555nm. In the sequential injection operation, the sample and the reagent are aspirated and mixed by reverse flow. The sequencing...
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