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A novel nucleic acid molecular 'light switch' method is developed for the sensitive recognition and detection of a single-base mismatched oligonucleotides. The detection limit of oligonucleotide of perfect double stranded and that with single-base, two-base and three-base mismatched are 0.11, 0.17, 0.34 and 1.5 ng ml -1 , respectively. It was found that Ru(phen) 2 (dppx) 2...
A simple reverse flow-injection (rFIA) manifold for the direct determination of aluminum in drinking water is proposed. This rapid and sensitive method is based on the formation of an Al 3+ complex with salicylaldehyde picolinoylhydrazone (SAPH), which shows a maximum blue-green fluorescence (λ ex =384 nm, λ em = 468 nm) at pH 5.4. Operative conditions both for...
A flow-through fluorescence bulk optode for the flow-injection determination of perchlorate is described. As the active constituents the optode incorporates the lipophilized pH indicator fluorescein octadecyl ester and methyl tridodecyl ammonium chloride, dissolved in a plasticized poly (vinyl) chloride membrane entrapped in a cellulose support. The optode is applied in conjunction with the flow injection...
A novel fluorimetric method for the determination of hemoglobin (Hb) using spiro form rhodamine B hydrazide (RBH) as fluorogenic reagent in the presence of sodium dodecylbenzene sulfonate (SDBS) surfactant micelles is described. The method employs the reaction of Hb with RBH, a colorless, non-fluorescent spirolactam hydrazide in SDBS micelles to generate highly fluorescent product rhodamine B and...
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