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A novel fluorimetric assay for dopamine using calcein blue (CB) complexed with Fe 2+ ion as a chemical sensor is described. The fluorescence arising from CB of the CB–Fe 2+ complex is quenched by the Fe 2+ ion. When dopamine is added to a solution of the CB–Fe 2+ complex, a dopamine–Fe 2+ complex is formed as the result of a ligand exchange reaction between...
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