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A functional nucleic acid-based amplification machine was designed for simple and label-free ultrasensitive colorimetric biosensing of microRNA (miRNA). The amplification machine was composed of a complex of trigger template and C-rich DNA modified molecular beacon (MB) and G-rich DNA (GDNA) as the probe, polymerase and nicking enzyme, and a dumbbell-shaped amplification template. The presence of...
DNAzyme-based catalytic beacons have been widely studied for both in vivo and in vitro molecular detection. However, only a few label-free catalytic beacons with excellent analytical performance have been reported so far. In this work, by combining a catalytic DNAzyme for amplified sensing through enzymatic turnover with cleavage-induced G-quadruplex formation, a label-free DNAzyme biosensor was developed...
Aptamers are analogous to antibodies in their range of target recognition. G-quadruplex DNAzymes exhibit peroxidase-like activity toward certain specific reactions. Despite aptazyme sensors, based on aptarmer and DNAzyme conjugates, have the potential to replace many conventional immune-biosensors; the mechanism concerning high background interference has scarcely been discussed. In this work, by...
A novel signal amplification strategy based on Cu2+-dependent DNAzyme was developed for sensing Cu2+ ion by combining hybridization chain reaction (HCR) with fluorescence resonance energy transfer (FRET) technique. In the presence of Cu2+ ion, the substrate strands of Cu2+-dependent DNAzyme immobilized on magnetic beads were specifically cleaved and released. The released strands initiated the HCR...
Recently a covalent peroxidase-mimicking DNAzyme (cPMDNAzyme) with the improved catalytic activity was prepared. Here we demonstrate that hydrogen peroxide, the oxidant substrate of cPMDNAzyme is an inactivating agent of this catalyst. Presence of the reductant substrate, 2,2′-azino-bis(3-ethylbenthothiazoline-6-sulfonic acid (ABTS) prevents the inactivation of cPMDNAzyme. The experimental conditions...
A novel label-free electrochemical biosensor for the detection of Hg2+ based on ligase mediated creation of G-quadruplex-hemin DNAzyme has been developed. Firstly, Cp probe was immobilized on the gold electrode surface through Au–SH bond. In the presence of Hg2+, Cp and Ap probes were partly hybridized with the LJ probe respectively through the specific T–Hg2+–T interaction. Then, the adjacent 3′-OH...
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