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In this paper, we have described a general strategy to facilely design ratiometric electrochemical sensors in electrolyte solutions, directly using a bare electrode for dual-signal sensing of analytes. Two types of substances (methylene blue/MB, doxorubicin/DOX) with different electrochemical signal peaks were added into electrolyte solutions (phosphate buffered saline, NaCl), where one was the analyte...
In this article, a facile aqueous synthesis of carbon dots (CDs) was developed by using natural kelp as a new carbon source. Through hydrothermal carbonization of kelp juice, fluorescent CDs were prepared and the CDs’ surface was modified with polyethylenimine (PEI). The PEI-modified CDs were conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites. To exploit broad applications,...
It is crucial to monitor intracellular pH values and their fluctuation since the organelles of cells have different pH distribution. Herein we construct a new small molecule fluorescent probe HBT-O for monitoring the subtle pH values within the scope of neutral to acid in living cells. The probe exhibited good water solubility, a marked turquoise to olivine emission color change in response to pH,...
Hydrogen sulfide (H2S) is a kind of gaseous signalling molecule that plays pivotal role in various biological processes. So far, it is still a challenge to develop convenient and reliable methods for H2S detection in lysosomes. Herein, we developed a novel ratiometric two-photon fluorescent probe LR-H2S for imaging H2S in lysosomes. Upon the addition of H2S (using Na2S as a donor) to LR-H2S in buffer...
Herein, a ratiometric turn-on fluorescent probe for sensitive detection of biothiols was designed. The probe consisted of two parts: one was rhodamine B serving as a fluorescence reference, and the other was coumarin derivative as the responsive fluorophore with an acrylate group for biothiols recognition. The response was based on the mechanism of Michael addition and intramolecular cyclization reaction,...
In this article, a facile and green synthesis of carbon dots (CDs) was developed by using natural carrot as new carbon source. After direct hydrothermal carbonization for 5h at 180°C, CDs were prepared facilely. Then, CDs were conjugated with polyethyleneimine (PEI) and Nile Blue (NB) chloride to produce CDs/PEI/NB nanocomposites under electrostatic interactions. Upon excitation at 800nm, two-photon...
A new ratiometric fluorescent probe based on a hemicyanine dye was synthesized. Investigation on the response behavior towards amino acids showed that the probe had a capability of rapidly and selectively detecting cysteine over other biothiols such as glutathione and homocysteine with similar structure and reactivity, and it also displayed a high selectivity and rapid response. Moreover, the probe...
A novel pH fluorescent probe 1-(pyren-1-yl)-3-(6-methoxypridin-3-yl)-acrylketone, (PMPA), which had a pyrene structure attached to methoxypyridine, was synthesized for monitoring extremely acidic and alkaline pH. The pH titrations indicated that PMPA displayed a remarkable emission enhancement with a pKa of 2.70 and responded linearly to minor pH fluctuations within the extremely acidic range of 1...
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