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An experiment was developed as a simple alternative to existing analytical methods for the simultaneous quantitation of glucose (substrate) and glucuronic acid (main product) in the bioprocesses Kombucha by using FTIR spectroscopy coupled to multivariate calibration (partial least-squares, PLS-1 and artificial neural networks, ANNs). Wavelength selection through a novel ranked regions genetic algorithm...
Despite wide application of cellulose-azure as a substrate for measuring cellulase activity, there is no quantification of hydrolysis rate or enzymatic activities using this substrate. The aim of this study was to quantify the hydrolysis rate in terms of product formation and dye released using cellulose-azure. The amount of dye released was correlated with the production of glucose and the enzyme...
A novel copper incorporated self-assembled monolayers (SAMs) modified gold electrode (Cu/SAMs) for determination of glucose was developed by electrodepositing Cu particles on the SAMs of hexanethiol. The scanning electron microscopic (SEM) images showed that copper formed orbicular particles of nanosizes on the SAMs, which was much different from the fractal-like particles of copper formed at gold...
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