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Enzyme-coupling reactions play an important role in the assay of analytes. In this manuscript, we developed a new fluorescent probe for the detection of glucose through the enzyme-coupling reaction of DT-diaphorase (DTD). The probe was synthesized through a mild and simple synthetic procedure, and showed good fluorescence response to DTD. The reactions for the detection of glucose proceed as follows:...
Prussian blue nanoparticles (PB NPs) exhibits an intrinsic peroxidase-like catalytic activity towards the hydrogen peroxide (H 2 O 2 )-mediated oxidation of classical peroxidase substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt to produce a colored product. The catalysis follows Michaelis–Menen kinetics and shows strong affinity for H 2 O ...
Partial Least Squares (PLS) regression is one of the most used methods for extracting chemical information from Near Infrared (NIR) spectroscopic measurements. The success of a PLS calibration relies largely on the representativeness of the calibration data set. This is not trivial, because not only the expected variation in the analyte of interest, but also the variation of other contributing factors...
A kind of dealloyed nanoporous gold (NPG)/ultrathin CuO film nanohybrid for non-enzymatic glucose sensing has been prepared by a simple, in-situ, time-saving and controllable two-step electrodeposition. The three-dimensional and bicontinuous nanoporous structure of the nanocomposites have been characterized by scanning electron microscope (SEM) and transmission electron microscopy (TEM), and the electrochemical...
Different materials like glass, silicon and poly(methyl methacrylate) (PMMA) are being used to immobilise enzymes in microchannels. PMMA shows advantages such as its low price, biocompatibility and attractive mechanical and chemical properties. Despite this, the introduction of reactive functional groups on PMMA is still problematic, either because of the complex chemistry or extended reaction time...
Well-distributed hollow gold nanospheres (Au shell @GOD) (20±5nm) were synthesized using the glucose oxidase (GOD) cross-linked with glutaraldehyde as a template. A glucose biosensor was prepared based on Au shell @GOD nanospheres for catalyzing luminol electrogenerated chemiluminescence (ECL). Firstly, chitosan was modified in a glassy carbon electrode which offered an interface of...
A facile fluorescence biosensor for the detection of glucose is proposed based on the pH-induced conformational switch of i-motif DNA in this paper. Glucose can be oxidized by oxygen (O 2 ) in the presence of glucose oxidase (GOD), and the generated gluconic acid can decrease the pH value of the solution and then induce the fluorophore- and quencher-labeled cytosine-rich single-stranded DNA...
By introducing p-phenylenediamine (PPD) to the hybrid system of Mn-doped CdS/ZnS quantum dots (QDs) and glucose oxidase (GOD), a sensitive label-free method was proposed for direct detection of glucose. With glucose and PPD as substrates, 2,5-diamino-N,N′-di-(4-aminophenyl)-2,5-cyclohexadiene-1,4-diimine (DDACD) that intensively quenches the fluorescence of QDs can be produced by the catalysis of...
Enzyme mimetics have recently attracted considerable interest because of their high stability and low cost. We developed a general H 2 O 2 -involved fluorescence system using Fe 3 O 4 magnetic microspheres as peroxidase mimetics and benzoic acid (BA) as indicator. Glucose and p-nitrophenol were used as models to determine the characteristics and effectiveness of the...
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