The effect of 8MeV electron irradiation on the magnetic and photoluminescence properties of Bi2Fe4O9 has been investigated and reported in this paper. Magnetic parameters of unirradiated and irradiated Bi2Fe4O9 nanoparticles were investigated by Vibrating sample magnetometer (VSM). Modification in saturation magnetization, remanence magnetization and coercivity were observed after exposure of Bi2Fe4O9 nanoparticles to high energy electrons. A decrease in the intensity of photoluminescence (PL) spectra was observed in irradiated samples compared to the unirradiated samples.