An easy one-pot polylol approach has been reported on the preparation of graphene decorated with magnetic Fe 3 O 4 nanoparticles for immobilizing porcine pancreatic lipase. The microstructures and interaction of the hybrids were characterized by several methods, which indicated that the Fe 3 O 4 nanoparticles are chemically bonded onto graphene sheets with tunable size and density. The graphene/Fe 3 O 4 hybrids provide excellent efficiency in immobilizing porcine pancreatic lipase, though dependent on pH value, temperature and denaturant. The porcine pancreatic lipase immobilized on graphene/Fe 3 O 4 hybrids becomes recyclable and have a remarkable thermal-stability in comparison with a free porcine pancreatic lipase. Our work demonstrates that graphene/Fe 3 O 4 hybrids could be high-quality and novel carriers for immobilizing lipase, which could be a good candidate for lipase immobilization.