A rapid and robust multiresidue method was developed for the analysis of seven quinolones (ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, and flumequine) in animal muscle tissues by HPLC-FLD. The samples were extracted with phosphate aqueous solution and cleaned with HLB-SPE. The analytes were separated using formic acid aqueous solution-acetontrile system as mobile phase with a linear gradient elution program, and determined by programmable fluorescence detection. The linear range was 0.3–1000 μg l −1 with correlation coefficients (r) greater than 0.9989. The limits of detection were 0.1–0.3 μg kg −1 , and limits of quantification were 0.3–1.0 μg kg −1 . The mean recoveries for each analyte in chicken and pig muscles ranged from 70.4% to 105.8% with relative standard deviation below 9.3% at 1–100 μg kg −1 fortification levels. The method is convenient, fast, safe, sensitive, and accurate for determining residual quinolones in actual samples.