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Aspergillus niger glucoamylase GA I cDNA was inserted in between the yeast PGK promoter and terminator on plasmid pMA91. The resultant plasmid pMAG69 was introduced into Saccharomyces cerevisiae GRF18 by protoplast transformation. The A. niger GA I cDNA wad expressed efficiently under the control of PGK promoter and 99% of the gene products were secreted into the culture medium using its own signal sequence. The recombinant yeast can digest 87% of starch in 2 d in the medium containing 10% starch. The recombinant plasmid pMAG69 can exist stably in S. cerevisiae.