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d-(−)-2-(6′-Hydroxy-7′-[123I]iodobenzothiazolyl)-Δ2-thiazoline-4-caroxylic acid (7′-[123I]iodo-d-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7′-Iodo-d-luciferin, a nonradioactive standard, was synthesized and showed the binding property (KM=4.28 μM) similar to that of d-luciferin (2.53 μM) for firefly luciferase in luminescence assay.
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