In vitro dissolution experiments although perhaps not at typical body concentrations and temperatures demonstrated that the α-hydroxycarboxylic acids present in interstitial fluid (citric acid, lactic acid, and malic acid) are capable of dissolving aluminum-containing adjuvants. Amorphous aluminum phosphate adjuvant dissolved more rapidly than crystalline aluminum hydroxide adjuvant. Intramuscular administration in New Zealand White rabbits of aluminum phosphate and aluminum hydroxide adjuvants, which were labelled with 2 6 Al, revealed that 2 6 Al was present in the first blood sample (1h) for both adjuvants. The area under the blood level curve for 28 days indicated that three times more aluminum was absorbed from aluminum phosphate adjuvant than aluminum hydroxide adjuvant. In vivo studies using 2 6 Al-labelled adjuvants are relatively safe because accelerator mass spectrometry (AMS) can quantify quantities of 2 6 Al as small as 10 - 1 7 g. A similar study in humans would require a whole-body exposure of 0.7μSv per year compared to the natural background exposure of 3000μSv per year. The in vitro dissolution and in vivo absorption studies indicate that aluminum-containing adjuvants which are administered intramuscularly are dissolved by α-hydroxycarboxylic acids in interstitial fluid, absorbed into the blood, distributed to tissues, and eliminated in the urine.