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A Tn5-derived mobile element has been constructed to identify genes and promoters related to pathogenesis and virulence inPseudomonas syringaepv.phaseolicola.To enhance the rate of mutation this Tn5derivative was constructed carrying a mutant transposase which was placed incisto the transposable element, but just outside the inverted repeats, therefore eliminating secondary transposition and increasing...
A set of low copy number plasmid vectors for mammalian gene expression has been constructed. These vectors are derived from the previously described bacterial low copy number expression vectors, pWSK29 and pWKS30, which are present at six to eight copies per cell. The new plasmids also have the following useful properties: (1) they contain antibiotic resistance markers for the selection of stable...
A new insertion sequence, IS1491,has been cloned and sequenced. The 2489-bp IS1491was isolated from aPseudomonas alcaligenesNCIB 9867 (strain P25X) 4.8-kbPstI chromosomal fragment. IS1491is flanked by an imperfect inverted repeat of 23 bp and carries two overlapping open reading frames, ORF1 and ORF2. Both ORF1 and ORF2 displayed homology to the IstA-like and IstB-like transposases encoded by the...
Shuttle vector pAT18 was transferred by conjugation fromEscherichia coliS17-1 toEnterococcus faecalisOG1RF andEnterococcus faeciumSE34. Transfer was mediated by the transfer functions of plasmid RK2 inE. coliS17-1 and the origin of conjugal transfer (oriT) located on pAT18. TheoriTsequence was then inserted into two plasmids to generate vectors pTEX5235 and pTEX5236. These two vectors cannot replicate...
The analysis of the complete nucleotide sequence of the small resistance plasmid pIE1107 revealed a close similarity to the well-known IncQ plasmids. Highly conserved replication proteins and nearly identical origins of replication (oriV) suggest equivalent functions in the related replication systems. However, pIE1107 contains two copies of IncQ-oriV-like DNA which are slightly different regarding...
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