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The pBG1 replicon from the cryptic plasmid ofDesulfovibrio desulfuricansG100A was inserted into pTZ18U derivatives to generate a new family of shuttle vectors. These plasmids are stable both inEscherichia coliand inDesulfovibrio,they present a large number of unique restriction sites, and colonies of recombinant clones can be identified by blue/white screening inE. coli.The pBMC, pBMK, and pBMS series...
A strain of Aeromonas hydrophila producing copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate, abbreviated as PHBHHx, was successfully transformed by electroporation. The plasmid used was a broad host range plasmid pBBR1MCS. Electroporation conditions were varied systemically to develop an electroporation protocol. The optimal yield of transformant was approximately 4×10 2 CFU/μg DNA...
Bacillus coagulans is a sporogenic lactic acid bacterium that ferments glucose and xylose, major components of plant biomass, a potential feedstock for cellulosic ethanol. The temperature and pH for optimum rate of growth of B. coagulans (50 to 55°C, pH 5.0) are very similar to that of commercially developed fungal cellulases (50°C; pH 4.8). Due to this match, simultaneous saccharification and fermentation...
Previously reported techniques for the electrotransfer of foreign DNA into pediococci yield only a small number of transformants/μg DNA, especially when using undomesticated strains. This study reports an improved protocol for the electrotransformation of pediococci, based on trials using Pediococcus acidilactici P60 and the plasmid pRS4C1. The improved protocol yields from 2 to 3 log units more transformants...
The pCB42 plasmid from Leuconostoc citreum CB2567, a strain isolated from kimchi, was characterized, and a shuttle vector for Escherichia coli and lactic acid bacteria (LAB) was constructed. The pCB42 plasmid has a circular structure of 4312bp, a low G+C content, and no single-stranded DNA intermediates during replication, which indicates that pCB42 replicates via the theta-type replication mechanism...
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