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Green fluorescent protein (GFP) is the most potential useful marker for the in situ monitoring of biofilm microbes. The objective of this study was to construct and compare the efficacy of transposon vectors containing native and foreign promoters in monitoring the denitrifying bacterium Pseudomonas stutzeri LYS-86 by chromosomal-integrated gfp. The promoter of nitrite reductase (Pnir) was cloned...
In utero electroporation (IUE) is a simple and rapid approach to in vivo investigate exogenous gene function in mouse brain, and intensive studies using IUE have greatly contributed to analyze the characterization of specific steps during mouse brain development. Because the efficiency of IUE is highly dependent on the plasmid used and its concentration, and the transcriptional activity of plasmid...
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